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> 한국미생물생명공학회(구 한국산업미생물학회) > Journal of Microbiology and Biotechnology > 19권 1호

Characterization of Chryseobacterium aquaticum Strain PUPC1 Producing a Novel Antifungal Protease from Rice Rhizosphere Soil

Characterization of Chryseobacterium aquaticum Strain PUPC1 Producing a Novel Antifungal Protease from Rice Rhizosphere Soil

( M. Gandhi Pragash ) , ( K. Badri Narayanan ) , ( P. Ravindra Naik ) , ( N. Sakthivel )

- 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)

- 발행년도 : 2009

- 간행물 : Journal of Microbiology and Biotechnology, 19권 1호

- 페이지 : pp.99-107 ( 총 9 페이지 )


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초록(외국어)
Strain PUPC1 produces an antifungal protease as well as plant growth promoting enzymes such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase and phosphatase. Morphological, cultural, and physiological characteristics as well as 16S rRNA gene-sequence-based phylogenetic analysis confirmed the taxonomic affiliation of PUPC1 as Chryseobacterium aquaticum. The optimum growth of PUPC1 was observed at pH 6.0 and 30℃, and maximum protease production was observed in medium B amended with 1% tryptone, 0.5% sucrose, and 0.005% MnCl2. The protease was purified by ammonium sulfate precipitation, Sephadex G-75 gel filtration chromatography, and electroelution from preparative SDS-PAGE. The protease had a molecular mass of 18.5 kDa. The optimum pH and temperature stability of the protease were pH 5.0-10.0 and temperature 40-70℃. Chryseobacterium aquaticum PUPC1 and its protease showed a broad-spectrum antifungal activity against phytopathogenic fungi. Strain PUPC1 also exhibited plant growth promoting traits. The objective of the present investigation was to isolate a strain for agricultural application for plant growth promotion and biocontrol of fungal diseases.

논문정보
  • - 주제 : 자연과학분야 > 생물
  • - 발행기관 : 한국미생물생명공학회(구 한국산업미생물학회)
  • - 간행물 : Journal of Microbiology and Biotechnology, 19권 1호
  • - 발행년도 : 2009
  • - 페이지 : pp.99-107 ( 총 9 페이지 )
  • - ECN : ECN-0102-2009-470-020201709
  • - UCI : I410-ECN-0102-2009-470-020201709

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저널정보
  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1017-7825
  • - 수록범위 : 1991–2017
  • - 수록 논문수 : 4824