Objectives: There are individual differences in the dissociative experience. The Authors approach the question about whether this difference is nature or nurture. The present investigation sought to explore the relationship between personality trait and dissociation. Method: Seventy-nine Korean university students(16 males and 63 females) had completed DESK(Dissociative Experience Scale-Korean version) to examine the ability of dissociation, Eye-roll Sign which is suggested the biological marker of dissociative ability by Spiegel, and TCI-K(Temperament and Character Inventory-Korean version) in order to evaluate personality trait. We divided the students into two groups(high DES group≥20 and low DES group<20) to evaluate the differences in TCI-K. Pearson`s correlation test, t-test and regression analysis were used for statistical analysis. Result: 1) In high DES-K group, score of self-directedness in TCI-K is higher than low DES-K group. There were statistically significant correlations between self-transcendence in TCI-K and amnesia subscale, absorbtion-imaginative involvement subscale in DES-K and total DES-K scale. Scores of self-transcendence in TCI-K was predicted for DES-K scores. 2) In high DES group, scores of Eye-roll Sign and squint subscale were higher than low DES group. There was no correlation between DES-K and Eye-roll Sign except weak correlation between depersonalization-derealization subscale in DES-K and gaze subscale in Eye-roll Sign. 3) There were statistically significant differences in TAS-K(Tellegan Absorbtion Scale-Korean version) and NHQ(Natural Hypnotic Experiences Questionnaire) between high DES-K group and low DES-K group. Also, there were statistically significant correlations between TAS-K and NHQ scale and DES-K. Conclusion: Although character dimension of TCI-K was related with dissociation, there was no difference in temperament dimension of TCI-K, and no correlation between DES-K and Eye-roll Sign. These results suggests that trait theory of dissociation is questionable and needs more investigations.