Purpose: The purpose of this study was to investigate the relations between fatigue and work-related factors in workers. Methods: A total of 204 subjects aged between 21 and 59 were selected through convenient sampling. Data were collected with a self-reported questionnaire from May 20 to 31, 2011. Collected data were analyzed with SPSS/WIN 17.0. Results: Difference in fatigue according to general characteristics was significant according to duty type. Difference in fatigue according to occupational characteristics was significant according to work intensity, work speed, demand of work concentration, work space, physical environment, largest weight by hands, vibration, visual demand of work, difficulty of work practice, work stress, and rest hours in holiday. There was a positive correlation between fatigue and occupational characteristics. Stepwise multiple regression analyses showed that educational level, work intensity, and rest hours in holiday were the strongest factors associated with fatigue (R2=.363 p<.001). These factors explained fatigue by 36.3%. Conclusion: The findings of this study may be useful in understanding workers` fatigue and developing more specific fatigue relief programs.