부산 송정 연안에서 분해중인 해조류를 채집하여 cellulose 분해 미생물을 분리·동정하고 미생물의 생육조건 및 미생물이 생성한 조효소의 cellulose 분해 특성을 확인하였다. Grateloupia elliptica로부터 분리한 cellulose 분해균을 동정한 결과, Cellulophaga lytica strain로 확인되었으며, Cellulophaga lytica PKA 1005 명명하였다. C. lytica PKA 1005의 최적생육 조건을 확인한 결과, pH 7, 2% NaCl, 30℃ 및 배양 36시간에서 최적생육활성을 확인하였다. 또한 C. lytica PKA 1005가 생성하는 cellulose 분해 조효소는 pH 8, 35℃, 8% CMC 및 반응 60시간에서 최적분해활성을 보이는 것을 확인하였다.
This study was conducted to investigate optimum conditions for the production of cellulose-degrading crude enzymes by an isolated marine bacterium. A marine microorganism producing an extracellular cellulose-degrading enzyme was isolated from the red seaweed, Grateloupia elliptica Holmes. The isolated bacterium was identified as Cellulophaga lytica by 16S ribosomal RNA gene sequence analysis and physiological profiling and designated as Cellulophaga lytica PKA 1005. The optimum conditions for the growth of Cellulophaga lytica PKA 1005 were pH 7, 2% NaCl, and 30oC with 36 h incubation time. To obtain the crude enzyme, the culture medium of the strain was centrifuged for 30 min at 12,000 × g and 4oC, and the supernatant was used as crude enzyme. The optimum conditions for the production of the cellulose-degrading crude enzyme were pH 8, 35oC, 8% carboxyl methyl cellulose, and 60 h reaction time.