This study is conducted on the “interpretational expression” of the “Palermo Annunciation” by Antonello da Messina depicting the ``Annunciation`` described in the Gospel according to Luke. Basically three elements appear in the iconography of the ``Annunciation`` in general: the angel, Mary, and the dove of the Holy Spirit coming down from the sky towards Mary. However, the “Palermo Annunciation” by Antonello da Messina did not include all of these basic elements of the iconography of the Annunciation. He drew only the Virgin Mary. Antonello clearly expressed the individuality of the artist when he interpreting the contents of the Bible in his reproduction. He tried to do something new, apart from the traditional iconography concept. In his painting, there are four time sequences, shaking, pondering, questioning, and obedience as he followed time sequence of the ``Annunciation`` of Luke’s gospel . He combined the various moments of time into one in one panel. He dynamically incorporated this diversity under one umbrella as a complete story, while not paying attention to the various elements of dissonance. A descriptive writer matches the behavior when he configures the storyline depending on the flow of time. The painter, Antonello, explained the story of the ``Annunciation`` from Luke’s Gospel in the Bible while connecting the acts in accordance with the flow of time with one small panel. Without the description that has already been created by the Evangelist; in other words, without the story of the “Annunciation”, the Antonello’s portrayal would not be able to connect a dissonance of time in human understanding. Moreover, the Evangelist Luke``s story would not have been possible if Mary had not understood herself as a human being in the storyline in the history of salvation.