In this study, we investigated anti-neuroinflammatory activities of enzymatic extracts of the microalgae residues, which were derived from the lipid extraction process, to improve the economic feasibility of algal biofuel. After lipid extraction, three microalgal residues (Chlorella sp., Nannochloropsis sp., Phaeodactylum tricornutum) were enzymatically hydrolyzed by four carbohydrases (Celluclast 1.5 L, Dextrozyme DX 1.5X, Promozyme D2, and Viscozyme L). Antioxidative activities of various enzymatic extracts from algal residues evaluated by measuring 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical-scavenging activities. All enzymatic extracts had scavenging potencies to ABTS + and alkyl radicals. Among the enzymatic extracts, Celluclast 1.5 L extracts from Nannochloropsis sp. residues (CN) showed the highest inhibitory activities on LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in murine BV-2 microglia, and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dosedependentmanner. CN also significantly reduced the generation of proinflammatory cytokines, such as interleukin-6 (IL-6), interleukin- 1β (IL-1β), and tumor necrosis factor (TNF)-α in a dose-dependent manner. Hence, these antioxidant and anti-neuroinflammatory effects of CN might assist potential of microalgae residues as nutraceuticals for treatment and prevention against neurodegenerative diseases that are accompanied by microglial activation.