The effects of milled chitosan solutions on growth and selected quality of soybean sprouts were evaluated.Chitosan solution (1% in 1% acetic acid) was milled for 0, 0.5, 1 and 2 h, and diluted with distilled water to 0.05% to use as soaking solutions. Soybean seeds were soaked in water (control), 0.05% acetic acid and milled chitosan solutions for 8 h, and then cultivated for 3 days at 25 o C. Results indicated that chitosan treatment increased total weight by 7.8-10.0% and hypocotyl length by 6.2-11.1% compared with the control and that the effectiveness of chitosan treatment was not affected by milling times of chitosan solution. Chitosan treatment, irrespective of milling times, did not affect the quality of soybean sprouts. No significant differences in moisture content, hardness and DPPH radical scavenging activity were observed among six treatment groups. The total free amino acid contents of soybean sprouts treated with 0.5 and 1 h-milled chitosan solutions were comparable to that of the control soybean sprouts.