As a interrogative pronoun in Mandarin Chinese, “nali” is often used for queries of location, but it’s also used as negation. This paper analyzed negative usage of “nali” from its structure form, and explored structural characteristics and conditions on negative usage of “nali”. The paper explored pragmatic negation of “nali” on linguistic factors which included intonation; sentence types, discourse and syntactic structures. The syntactic structures include the syntactic position of “nali” and verb categories collocated with “nali”. Using of its situation and social status of communicators were discussed as well. Pragmatic negation of “nali” can used as the expression of subjective and objective which are different from “bu” and “mei (you)”.