The purpose of this study is to analyze workplace communication conflicts with respect to position and gender. Communication conflicts arising from work performance are known to cause emotional difficulties in the workplace, which forms a significant part of an adult`s everyday life. To investigate workplace communication conflict, the study solicited written interviews from eighteen office workers and seven job consultants, asking about conflict suffered during work performance. For qualitative analysis, work relationships were identified by position (seniors, same level, and juniors) and gender (male-female, male-male, and female-female). Interview data were analyzed through NVIVO 11 program. With respect to position, the study found that the greatest communication conflicts involved seniors communicating with others, with the most frequently cited causes being “unreasonable instructions” and “ignorance of opinions.” Between workers at the same level, most conflicts came from failures to keep confidences. The greatest source of communication conflicts with juniors was their largely negative reactions to instructions. With respect to gender, the study found that the greatest sources of communication conflict related to differences in job perception between males and females and confusion of subordinate-superior relationships between the same genders. Also, the female-female dynamic was found to have a unique set of communication conflicts.