Purpose: The purpose of this study was to identify the level of sexual assertiveness in women students in nursing and also, to investigate factors that influence sexual assertiveness. Methods: In this study, 373 women students in nursing who were enrolled in a university in B city, Korea and who gave written consent completed the questionnaire between May 15 and May 30, 2016. Data were analyzed using t-test, one-way ANOVA, Pearson correlation coefficients and multiple regression. Results: The level of sexual assertiveness in the women students in nursing was 54.84±5.82. Sexual assertiveness showed negative correlations with gender role stereotypes (r=-.37, p<.001), and permissiveness of dating violence (r=-.15, p<.005), and a positive correlation with self-esteem (r=.36, p<.001). Models including the variables explained 18% of the variance for sexual assertiveness. Gender role stereotypes and self-esteem were the influential factors. Conclusion: Based on the outcomes of this study, it is necessary to design intervention programs that emphasize sexual assertiveness of women students in nursing.