Hyperthermal acid hydrolysis pretreatment of Eucheuma denticulatum was carried out using 12% (w/v) seaweed slurry and 90 mM H₃PO₄ at 150℃ for 10 min. The use of Candida lusitaniae with adaptive evolu-tion was evaluated for ethanol fermentation. The levels of ethanol production by separate hydrolysis and fermentation (SHF) at 72 h with non-adapted and adapted C. lusitaniae were 10.1 g/l with ethanol yield (Y_EtOH) of 0.23, and 18.1 g/l with YEtOH of 0.45, respectively. Adaptive evolution was employed in this study to improve the efficiency of ethanol fermentation. Development of the SHF process could enhance the overall ethanol fermentation yields of the red seaweed E. denticulatum.