An experimental investigation was performed to study the characteristics of laminar water flow in a horizontal circular tube by using liquid crystal. A simultaneous measurement technique has been employed to measure the velocity field in a two-dimensional cross section of fluid flow. This study found the velocity distributions for Re = 1,594~2,510 along longitudinal sections and the results appear to be physically reasonable. To determine some characteristics of the laminar flow, 2D PIV technique is employed for velocity measurement by using liquid crystal in water. The experimental rig was manufactured from an acryle tube. The test tube diameter of 25mm, and a length of 1200mm. The used algorithm is the gray level cross―correlation method by using Kimura et al. in 1986.