Modern biotechnological industries have been attempting to improve the efficiency of bacterial strain cultivation. Millimeter wave electromagnetic radiation can have a varied influence on E. coli cultivation processes. The results of the study revealed that when a microwave radiation of low intensity is applied to positively adjust the conditions for the accumulation of bacterial culture biomass, a significant role is played not only by radiation parameters, but also by concomitant biological factors, which influence the reproducibility of the cultivation process and help obtain a useful biotechnological effect. The authors suggest a model that can be used to study the molecular mechanisms underlying the changes in the buildup of E. coli biomass under the influence of electromagnetic radiation.