N-Methylcarbamate系 農藥의 殘留分析法을 確立하기 위하여 MIPC, XMC, BPMC, propoxur, carbofuran 및 carbary에 대하여 diazo化에 의한 比色定量과 N-trifiuoroacetyl (TFA)化, pentafluorobenzyl ether(PFB)化 및 dinitrophenyl ether (DNP)化에 의 한 誘導體의 gas chromatography (ECD)法을 比較檢討하였다.
1) N-Methylcarbamate의 diazo化에 의한 最大吸收波長은 MIPC, XMC, BPMC, propoxur, carbofuran이 460 ㎚이었고 carbaryl은 510 ㎚이었다.
2) 比色法에 의한 carbamate의 玄米와 사과中 回收率은 各各 80∼104%, 98∼120%이었고, 50 g의 試料分析時 最大檢出限界는 0.03∼0.05 ppm의 範圍이었다.
3) Gas chromatography에 의한 carbamate의 分析에서는 DNP
Analytical methods for residues of six N-methylcarbamate insecticides were investigated to compare the minimum detectability, recovery from several crops and feasibility of multiple residue analysis. Those methods studied in this work included spectrophotometry by diazotization and gas chromatography by N-trifluoroacetylation(TFA), pentafluorobenzylether(PFB) and dinitrophenylether (DNP) derivatization. Maximum absorbed wavelength of the diazotized MIPC, XMC, BPMC, propoxur and carbofu ran was around 460 ㎚, while that of carbaryl recorded 510 ㎚. Recovery from brown rice and apple by diazotization method ranged from 80 to 120% and minimum detectable limits were 0.03 to 0.05 ppm in 50 g of the sample. Minimum detectability of PFB derivatives by gas chromatography was superior to TFA and DNP derivatives. DNP derivatives showed the longest retention time among the given derivatives. Recovery from crops by gas chromatographic met hod ranged 74 to 94%, 78 to 93%, and 85 to 99% in brown rice, rice straw and apple, respectively. Limit of detection was 0.01 ppm for TFA, 0.005 ppm for PFB and 0.02 ppm for DNP derivatives in 50 g of the crop samples.