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논문검색은 역시 페이퍼서치

Genes & Genomics(구 한국유전학회지)검색

Korean journal of genetics


  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기: 격월
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : SCI / SCOPUS
  • - ISSN : 1976-9571
  • - 간행물명 변경 사항 : 한국유전학회지(~2007)→genes & genomics(2008~)
논문제목
수록 범위 : 30권 2호 (2008)

Research Articles : Overexpression of Zinc Finger Protein of Capsicum annuum(PIF1) in Tobacco Enhances Cold Tolerance

( Eul Won Hwang ) , ( Soo Chul Park ) , ( Myung Ok Byun ) , ( Mie Young Choi ) , ( Hawk Bin Kwon )
4,000
초록보기
Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper (Capsicum annuum) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances.

Research Articles : Molecular Cloning and Expression of the Mitochondrial 60-kDa Heat Shock Protein from an Arctic Copepod Calanus glacialis

( Il Chan Kim ) , ( Chang Eun Lee ) , ( Hyun Hee Cho ) , ( Soon Gyu Hong ) , ( Jin Sung Lee ) , ( Hyoung Seok Lee ) , ( Hong Kum Lee )
4,600
초록보기
Heat shock proteins (Hsps) play an essential role in cell defense against cell damages by several environmental and physiological stresses. We cloned and sequenced full-length heat shock protein 60-kDa (Hsp60) from the Arctic copepod Calanus glacialis cDNA. The complete nucleotide sequence of Calanus glacialis Hsp60 ( CgHsp60) gene contained 2,092 bp in length. The open reading frame (ORF) was 1,743 bp flanked by a 5`-UTR of 90 bp and a long 3`-UTR of 259 bp, and encoded a putative protein of 581 amino acids with a calculated molecular weight of 62 kDa. It showed high similarity to those of Culicoides variipennis(73%), Aedes aegypti (72%), and Tribolium castaneum (71%) at the amino acid sequence level. In phylogenetic analysis, it clustered with other arthropods sequences. We also confirmed that the recombinant protein of C. glacialis Hsp60 by the SDS-PAGE and immunoblotting analysis after expression of recombinant gene construct in Escherichia coli.
4,000
초록보기
To characterize the mitochondrial genome of the threespot wrasse Halichoeres trimaculatus (Perciformes, Labridae), we cloned and sequenced the complete mitochondrial DNA. The H. trimaculatus mitochondrial genome is 16,621 base pairs (bp) in length and consists of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control regions, as found in the mitochondrial genomes of other bony fishes. Most of the H. trimaculatus genes are encoded on the L-strand, whereas the nicotinamide adenine dinucleotide dehydrogenase (ND) 6 and eight tRNA (Gln, Ala, Asn, Cys, Tyr, Ser-UCN, Glu, and Pro) genes are encoded on the H-strand. Two reading-frame overlaps were found on the same strand: ATPase subunit (ATP) 8 and 6 overlapped by ten nucleotides, and ND4L and ND4 overlapped by seven nucleotides. The L-strand replication origin (31 bp) was located between tRNA-Asn and tRNA-Cys genes in the H. trimaculatus mitochondrial genome. The control region, which spans 861 bp, is located between tRNA-Pro and tRNA-Phe that shows consistency with its location as other bony fishes. Within this sequence, we identified several domain sequences such as a termination associated sequence and the conserved sequence blocks 1, 2, 3 as well as D box domains. These results provide basic information for comparative mitochondrial DNA in the genus Labridae along with further consideration on molecular phylogeny and biogeographic studies in the Halichoeres species.

Research Articles : Characteristics of Mitochondrial DNA Sequence Polymorphisms and Haplogroups in Korean Population

( Hye Ran Kim ) , ( Myung Geun Shin ) , ( Mi Ji Kim ) , ( Jong Hee Shin ) , ( Soon Pal Suh ) , ( Dong Wook Ryang )
4,000
초록보기
We examined sequence variations in the mitochondrial DNA (mtDNA) control region, tRNA leucine1 ( tRNA leu1) and cytochrome b ( CYTB) genes in order to investigate the characteristics of mtDNA polymorphisms and haplogroups in Korean population. Seventy maternally unrelated healthy Korean donors provided blood samples for the present study. The small deletion mutations exist only in the hypervariable region (HV) region. The Korean population exhibited a high level of length heteroplasmy in the 16184-16193 and 303-315 poly-C regions from the mtDNA control region. Some of the most common polymorphisms found in all subjects were 73A > G, 263A > G, 3107delC and 15326A > G from HV2, HV1, tRNA leu1 and CYTB genes, respectively. The most common haplogroup in the Korean population was D4 which was found in 16% of the population, followed by A, B, B4a, D5, G1a and M10 (each of 6%). Several haplogroups appear to be restricted to the Japanese and Korean populations. However, the current study revealed different distribution of some haplogroups in the Korean population in comparison with a previous study. The overall pattern and frequency of haplogroups among Koreans in the current study were closer to those of a Japanese population than to a Han Chinese population.

Research Articles : Morphometric Traits and Condition Indices in Artificially Induced Hybrids and Their Parental Species, Haliotis gigantean Gmelin (♀) and H. discus discus Reeve (♂)

( In Seok Park ) , ( Jun Wook Hur ) , ( Soo Yeon Im ) , ( Dong Won Seol ) , ( Woo June Hur ) , ( Min Ouk Park ) , ( Hye Suck An ) , ( Eun Mi Kim ) , ( Seok Jung Han )
4,000
초록보기
Morphometric traits, percent shell weight, percent cavity volume and condition index of 4-year-old induced hybrid abalone from female Haliotis gigantea Gmelin and male Haliotis discus discus Reeve were compared to those of the parental species. Apex height/shell height of the induced hybrid abalone was in between the average height of the parental species. The induced hybrid abalone shared three traits with the paternal species and two with the maternal species. After 4 years of growth, the condition index of the induced hybrids weresignificantly higher than that of either parental species ( P< 0.05). Generally, induced hybrid abalone displayed an external morphology that was intermediate between the representative of both parental species. Haliotis gigantea and the induced hybrids had four respiratory pores, while H. discus discus had five.

Research Articles : The Production and Cytological Analysis of Brassica napus-B Genome Chromosome Monosomic Addition Lines and Their Hybrids

( Mao Teng Li ) , ( Jun Xiang ) , ( Jian Min Liu ) , ( Long Jiang Yu ) , ( Dian Rong Li )
4,000
초록보기
The Brassica napus-B genome monosomic addition lines (MALs) (AACC + B`, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus-B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19II+1I, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20II and 19II+2I that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes.

Research Articles : Overexpression of Hoxc8, a Homeobox Gene, in B16F10 Melanoma Inhibits the Growth of Tumor Cells

( Byung Gyu Kim ) , ( Hyoung Woo Park ) , ( Kyung Ah Park ) , ( Myoung Hee Kim )
4,000
초록보기
Homeobox (Hox)-containing genes have been identified as regulators which control the expression of a variety of genes involved in development and differentiation. Recent reports also suggested an involvement of Hox genes in transformation and/or tumor progression. In human prostate cancer, overexpression of HOXC8 was associated with a loss of tumor differentiation, which implies the involvement of HOXC8 in the process leading to the tumorigenicity of tumor cells. In order to investigate the role of Hoxc8 in the growth of tumor cells, Hoxc8 was designed to be expressed constitutively in B16F10 melanoma cells after stable transfection, and then a clone B16F10Shoxc8#14 overexpressing Hoxc8 was selected and analyzed further. B16F10Shoxc8#14 expressed Hoxc8 at high level and exhibited a reduced growth rate in vitro. When the cell cycle progress was analyzed, it has a decreased S phase population through upregulation of cell cycle regulators, such as p21, HDAC8 and E2F5. When the effect of Hoxc8 was analyzed on tumor growth in vivo C57BL/6 mice, the tumorigenicity as well as the growth rate was significantly decreased, indicating that the overexpression of Hoxc8 in B16F10 melanoma inhibits the tumor proliferation, probably functioning as a cell cycle regulator.

Research Articles : The A190T Variation in Human Polycystin 2, Previously Known as a Missense Mutation, Is a Nonsynonymous cSNP in Korean Population

( Mi Hyun Park ) , ( Hye Kyung Kim ) , ( Jin Man Kim ) , ( Byung Ho Lee ) , ( Mi Hee Chang ) , ( Me Yae Lee ) , ( Hyeon Jin Kim ) , ( Jong Young Lee ) , ( Seong Chool Hong )
4,000
초록보기
Polycystin 2 (PKD2) is an ion channel that regulates the intracellular calcium ion concentration. Amino acid changes in PKD2 are known to cause autosomal dominant polycystic kidney disease (ADPKD), and are possibly associated with many other complex diseases. In an effort to find cSNPs (single nucleotide polymorphisms within cDNA sequence) in PKD2, we used direct sequencing analysis to screen the entire coding region of the PKD2 gene. We identified a major nonsynonymous cSNP, Ala190Thr (A190T), in the N-terminal intracellular domain of PKD2. This nonsynonymous cSNP was previously reported as a mutation for ADPKD. However, this study excluded the possibility that the A190T variation was a missense mutation for ADPKD by a thorough clinical evaluation and population study. In 500 genetically unrelated Koreans, the allele frequencies of G and A at the A190T variation were 64% and 36%, respectively. The genotype frequency of the cSNP was not deviated from Hardy-Weinberg equilibrium. These experimental results indicate that mutational research needs to be confirmed by a thorough clinical evaluation and population study. Furthermore, the A190T variation is implicated as having possible causative roles in the pathogenesis of various ADPKD-related diseases. Intensive population studies should be conducted to address the etiological role of the A190T variation in these ADPKD-related diseases.

Research Articles : Karyotype Analysis and Physical Mapping Using Two rRNA Genes in Dioecious Plant, Humulus japonicus Sieboid & Zucc

( Soo Young Kim ) , ( Chan Soo Kim ) , ( Joong Ku Lee ) , ( Jae Wook Bang )
4,000
초록보기
Using meiosis and mitosis chromosomes, we have karyotyped and physically mapped of Humulus japonicus. The somatic metaphase chromosome numbers were 2n = 2x = 14 + XX in female, 2n = 2x = 14 + XY1Y2 in male and 2n = 3x = 21 + XXX in triploid. The 5S and 45S rDNA genes were physically mapped on the metaphase chromosomes using fluorescence in situ hybridization (FISH). One pair of 5S rDNA gene was detected on the short arm of chromosome 4 and two pairs of 45S rDNA gene were observed on the terminal regions of the short arms of chromosome 5 and 7 in both female and male plants. In addition, three pairs of two rDNAs were detected on the chromosome 4, 5 and 7 of triploid. At meiosis, a trivalent chromosome figure was clearly seen, of which Y1 and Y2 could be distinguished from X by their premature condensation and three chromosomes, Y1-X-Y2, the forms associated by terminal chiasmata.

Research Articles : The Adenylyl Cyclase Encoded by rutabaga Is Required for Larval Molting in Drosophila

( Won Seok Son ) , ( Pyung Lim Han ) , ( Yun Doo Chung )
4,000
초록보기
Hormonal control of insect growth and metamorphosis by neuroendocrine system is well established, yet the cellular mechanism underlying the synthesis and release of insect hormones is not clearly understood. Here we report that a novel enhancer detection line, rutlethal-1096, failed to molt from second to third instar larvae, thus showing a lethal phenotype. Molecular cloning indicated that the enhancer detector was inserted in the proximal promoter region of the rut-encoded adenylyl cyclase (AC) gene. Similarly, the known rut alleles, rut1 and rut1084, were also found to be affected mildly in their larval and pupal development. The enriched expression of the reporter gene lacZ was detected in the ring gland that is an insect larval glandular organ secreting various hormones involved in ecdysis. Taken together, our data suggest the rut-encoded AC plays a vital role in larval molting, probably by regulating the function of ring glands.
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