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논문검색은 역시 페이퍼서치

한국미생물·생명공학회지검색

Microbiology and Biotechnology Letters


  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기: 계간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : - / SCOPUS
  • - ISSN : 1598-642x
  • - 간행물명 변경 사항 : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)
논문제목
수록 범위 : 39권 4호 (2011)

보문 : 유전자조작, 균주분리 ; 대한민국 대천 해안에서 분리한 전분 분해능을 갖는 Pseudoalteromonas sp. A-3 균주의 특징 및 동정

지원재 ( Won Jae Chi ) , 박다연 ( Da Yeon Park ) , 정성철 ( Sung Cheol Jeong ) , 장용근 ( Yong Keun Chang ) , 홍순광 ( Soon Kwang Hong )
4,500
초록보기
Amylase를 생산하는 능력을 갖고 있는 A-3 균주가 대한민국 대천 해변가의 바닷물로부터 분리되었다. A-3 균주는 1개의 polar flagella를 갖으며, Artificial Sea Water-Yeast extract-Peptone(ASW-YP) 한천배지 위에서 배양할 경우 20-37℃에서 잘 자라지만, 15℃와 40℃에서는 천천히 자라는 속성을 보였다. 또한 ASW-YP 액체배지를 사용하는 경우, pH 6-9 범위에서 잘 자라는 반면 pH 4-5, pH 10에서는 전혀 성장하지 못했다. 16S rRNA sequence 분석 결과, A-3 균주는 Pseudoalteromonas phenolica O-BC30T, Pseudoalteromonas luteoviolacea NCIMB1893T, Pseudoalteromonas rubra ATCC29570T, Pseudoalteromonas byunsanensis FR1199T와 각각 98.3, 97.86, 97.25%의 similarity를 보였으며, 이를 기초로 한 phylogenetic tree 분석결과, P. phenolica O-BC30T와 같은 clade를 형성하였다. 그러나, A-3 균주는 5% 이상의 Nacl 농도에서 전혀 성장하지 않고, D-glucose, D-mannose, D-maltose, D-melibiose를 이용하지 못하며, lipase 활성(C-14)이 없는 등 많은 생리학적 특성이 P. phenolica O-BC30T와는 상당히 달랐다. 이러한 생리학적 차이로부터 우리는 A-3 균주가 P. phenolica O-BC30T와는 다른 종으로 판단하고, 이 균주를 Pseudoalteromonas sp. A-3로 명명하였다. Pseudoalteromonas sp. A-3는 배양시기 동안 계속해서 안정적으로 α-amylase를 생산했으며, 총 amylase 활성은 pH 7과 37℃에서 최대값을 보였다. 이 amylase 활성은 pH 10까지도 비교적 안정적이었으며, 이러한 alkali-tolerant amylase는 산업적으로도 유용성이 클 것으로 사료된다.

보문 : 유전자조작, 균주분리 ; 갯메꽃 뿌리로부터 분리된 내생진균의 식물생장촉진활성

유영현 ( Young Hyun You ) , 윤혁준 ( Hyeok Jun Yoon ) , 우주리 ( Ju Ri Woo ) , 서영교 ( Yeong Gyo Seo ) , 신재호 ( Jae Ho Shin ) , 추연식 ( Yeon Sik Choo ) , 이인중 ( In Jung Lee ) , 김종국 ( Jong Guk Kim )
4,500
초록보기
Eight endophytic fungal strains were isolated from the roots of Calystegia soldanella from the western coast of South Korea. The culture filtrate of the eight endophytic fungi were applied to waito-c rice seedlings in order to verify potential plant growth promotion activities. The results of bioassay indicated that the Cs-9-7 fungal strain possessed the highest plant growht promotion activity. Fungal culture filtrates were analyzed to verify secondary metabolites using gas chromatography and mass spectroscopy with selected ion monitoring (GC/MS-SIM). The culture filtrate of the Cs-9-7 fungal strain was confirmed to contain gibberellins GA3 (1.229 ng/mL), GA4 (3.535 ng/mL), GA7 (1.408 ng/mL) and GA12 (0.378 ng/mL). Polymerase chain reactions (PCR) were performed so as to determine the internal transcribed spacer (ITS) regions for the identification of isolated strains with universal primers ITS-1 and ITS-4. The Cs-9-7 fungal strain, isolated from the root of C. soldanella, has been named Aspergillus tubingensis Cs-9-7.

보문 : 유전자조작, 균주분리 ; 간장 유래 혈전분해 효소 생산 균주의 분리 및 배양학적 특성

백성열 ( Seong Yeol Back ) , 윤혜주 ( Hye Ju Yun ) , 박희동 ( Heui Dong Park ) , 여수환 ( Soo Hwan Yeo )
4,500
초록보기
Bacterial strains exhibiting fibrinolytic activity were screened from traditional Korean soybean sauce. The Fibrinolytic activities of the various isolated microorganism were further examined and the superior strain YJ11-21 was selected for further analyses. Gene sequence analysis of 16S rDNA of the YJ11-21 strain revealed Bacillus lichenformis. Optimal culture conditions were investigated in order to maximize the production of the fibrinolytic enzyme by YJ11-21. Amongst the carbon sources tested, glucose was the most effective for enzyme production and amongst the nitrogen sources tested, yeast extract was seen to be the most effective. A one percent addition of NaCI to the medium resulted in the highest fibrinolytic activity. Interestingly, a 10% addition of NaCI resulted in a high activity together with a high cell growth rate. Therefore, YJ11-21 is speculated of being a halotolerant. The optimum pH and temperature for enzyme production were a pH of 9.0 and 30℃, respectively.

보문 : 유전자조작, 균주분리 ; 동물 세포 내에서 WJ1 인티그라제에 의한 부위 특이적 재조합

김혜영 ( Hye Young Kim ) , 윤보현 ( Bo Hyun Yoon ) , 장효일 ( Hyo Ihi Chang )
4,500
초록보기
이전 연구에서, bacteriophage ΦFC1이 Enterococcus faecalis KBL703에서 UV induction을 통해 분리 동정되었으며, ΦFC1은 phage attachment site인 attP와 bacterial attachment site인 attB 사이에서 site-specific integration을 촉매하는 integrase를 가지고 있다는 것을 밝혀냈으며 이를 MJ1이라 명명하였다. 이 연구에서는 이를 바탕으로 MJ1에 의한 site-specific integration의 효율을 Escherichia coli와 NIH3T3 cell에서 확인 하기 위해 attP, attB, MJ1을 각각의 벡터에 삽입하였다. MJ1 인테그라제에 의한 재조합을 수행하기 위해서 기질 벡터 pABLP를 DH5α에 형질전환시킨 후, LB 배지에서 37℃ l시간 배양한 후 암피실린(ampicillin)과 테트라싸이클린(tetracycline) 항생제 플레이트로 pGMJ1과 pABLP 같이 가지고 있는 colony 들을 선별하여, LacZ 유전자가 불활성화 된 흰색 콜로니 개수를 세고 통계를 낸 결과 integration의 frequency가 99% 이상인 것으로 나타났다. 또한, 실제로 재조합이 일어났는 지를 확인하기 위해서 콜로니 PCR을 수행하여 재조합의 산물인 attL 150 bp을 확인하였다. PCR 산물은 염기서열분석을 통해 정확한 site-specific integration이 일어났음을 확인하였다. MJ1에 의한 integration을 보이기 위해 attP와 attB를 가지고 있는 vector를 MJ1 expression vector와 함께 NIH3T3 cell에 cotransfection 했으며 GFP를 reporter로 사용해 그 activity를 관찰하였다. NIH3T3 cell에서 GFP의 발현을 형광 현미경을 통해 알아본 결과, MJ1에 의한 site-specific integration이 다른 accessory protein의 도움 없이 일어난다는 것을 볼 수 있었다. 마찬가지 방법으로, attR과 attL 간의 excision을 GFP로 알아본 결과, GFP는 발현하지 않았으며, 이는 MJ1에 의한 excision이 일어나지 않았음을 보여주었다. 이와 같은 결과로 볼 때, MJ1의 host만이 아니라 넓은 범위안에서도 integration을 수행할 수 있다는 것을 보여주었다. 따라서 MJ1을 이용한 site-specific integration system의 개발은 gene therapy를 위한 gene delivery system의 구축에 있어서 좋은 시작이 될 수 있다.

보문 : 발효, 세포배양, 생물공정 ; 백하수오 발효액의 모발성장 효과

박장순 ( Jang Soon Park ) , 이재숙 ( Jae Sug Lee )
4,500
초록보기
The bacterial growth and pH of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus during the fermentation process, were evaluated. The results indicated that the bacterial count after fermentation always remained higher than 6 log CFU/mL, with a constant pH of approximately 4. In order to evaluate the effects of Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, on hair growth promotion, C57BL/6 mice were chosen as experimental subjects. Six week old males with similar body weights were divided into four groups; a normal group (saline), a negative control group (essence base), a positive control group and an experimental group (Pleuropterus cilinervis extracts and animal milk, fermented with Lactobacillus rhamnosus, mixed with negative control). The substances and test materials were applied topically on the back skin of the mice for 8days. Other external conditions and variables such as food intake were kept at the same as the four groups. At the end of the experiment it was noted that hair re-growth in the experimental group, using gross and histological examinations, was higher than that in the positive control group. This study therefore provides an empirical evidence that Pleuropterus cilinervis extracts and animal milk fermented with Lactobacillus rhamnosus promotes hair growth, and suggests that applications could be found in hair loss treatments.

보문 : 발효, 세포배양, 생물공정 ; 유산균 발효에 의한 십전대보탕의 발효 전 후 성분 변화 연구

양민철 ( Min Cheol Yang ) , 정상원 ( Sang Won Jeong ) , 마진열 ( Jin Yeul Ma )
4,500
초록보기
The purpose of this study was to investigate changes in the contents of constituents in Sipjundaebo-tang (SJ) and its fermentations (FSJ) with 8 species of lactic acid bacteria. Eight strains of lactic acid bacteria, Lactobacillus casei KFRI129, L. plantarum KFRI 144, L. curvatus KFRI 166, L. hilgardii KFRI 229, L. delbruekil subsp. lactis KFRI 442, L. casei KFRI 692, L. gasseri KCTC 3163, and Bifidobacterium breve KFRI 744, were utilized for the fermentation of Sipjundaebo-tang. As a result we identified three constituents which increased and two constituents which decreased. The increased constituents were isolated by chromatographic techniques and then their structure elucidated using NMR and MS. The decreased constituents were confimed by comparing standard compounds. These compounds were quantitatively analyzed using the HPLC/DAD system. The increased constituents were identified to be cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5), while the decreased constituents were liquiritin (2) and nodakenin (4). Generally, liquiritin (2) and nodakenin (4) were noted as having decreased in fermented Sipjundaebo-tangs (FSJs), while cinnamyl alcohol (1), liquiritigenin (3), and nodakenetin (5) were seen to have increased. Sipjundaebo-tang fermented by L. plantarum KFRI144 exhibited the most remarkable changes in all of fermentations. The eight lactic acid bacteria all demonstrated differing decomposable rates on the five maker compounds in fermented Sipjundaebo-tang (FSJ).

보문 : 발효, 세포배양, 생물공정 ; Aspergillus 속 곰팡이를 이용한 액체종국 제조 및 밀누룩의 품질특성

최정실 ( Jeong Sil Choi ) , 정석태 ( Seok Tae Jung ) , 김주연 ( Joo Yeon Kim ) , 최지호 ( Ji Ho Choi ) , 최한석 ( Han Seok Choi ) , 여수환 ( Soo Hwan Yeo )
4,500
초록보기
This study focus was primarily the development of liquid starters for Aspergillus oryzae and Aspergillus niger prepared with wheat bran as a low cost culture medium. For the preparation of the liquid media wheat bran was added at rates of 0, 5, 10, 15 and 20% and the Aspergillus sp. strains were then inoculated to these prepared broths. The results indicated that the more that wheat bran was contained in the medium, the more mycelia was produced for A. oryzae and A. niger. The highest enzyme activities were obtained with a 10~15% adding rate of wheat bran for both strains. Changes in the enzyme activities of the liquid starters during various incubation times (0, 24, 48, 72 and 96 hrs), indicated that the highest enzyme activities were seen between 48 and 72 hrs of culture. In addition, a comparative study was carried out on the production of enzymes using wheat as a substrate in nuruk, with liquid starter made from fermented agents according to the same concentrations used with the wheat bran. The pH, acidity, amino acidity, reducing sugar content and enzyme activity (α-amylase, glucoamylase, acidic protease) of wheat nuruk made with liquid starter were compared with those of wheat nuruk made with solid starter. The results suggest that the liquid starter is superior in both cases.

보문 : 발효, 세포배양, 생물공정 ; 세포 크기 차이를 이용한 유세포 분석을 통한 인간배아줄기세포 유래 기능성 혈관세포의 확립

이지혜 ( Ji Hye Lee ) , 김주미 ( Ju Mi Kim ) , 정형민 ( Hyung Min Chung ) , 채정일 ( Jung Il Chae )
4,500
초록보기
인간배아줄기세포는 인간배아줄기세포가 가지는 전 분화능 등의 특이적 특성으로 인해 재생의학 분야에서 세포 치료제의 근원으로 널리 각광받고 있다. 그러나, 미분화 상태의 인간배아줄기세포를 세포치료제로 이용하기 위해서는 인간배아줄기세포 주 유래 기능성 세포를 확립이 반드시 요구된다. 본 연구에서는, 미분화 상태의 인간배아줄기세포 주로부터 기능성 세포의 확립을 위해, 혈관계통의 세포로 분화를 유도하였으며, 분화 유도 후 세포의 크기 차이를 이용하여 특정 세포군 만을 분리하여 그 기능성을 비교 분석하였다. 그 결과, VEGF를 이용하여 분화 시킨 세포군에서 약 10%의 PECAM 양성 세포군을 확인할 수 있었으며, 분리 및 세포 이식을 위해 세포를 단일 세포군으로 만들었다. 단일 세포군의 형성 후, 유세포 분석기를 이용한 세포 분리 기법을 이용하여 FCS를 기준으로 한 세포 크기의 차이를 이용하여 특정 세포군 만을 분리하여, 하지 허혈 동물 모델로의 이식을 통해, 비 분리 세포군과 치료 효능을 비교 분석을 실시하였다. 세포 이식 4주 후, 혈류량 복구율이 FSC 기준 분리 군의 경우 54%, 비 분리군의 경우 l7%를 보이는 것을 확인하였다. 이 결과는, 초기 분화 유도 후 세포 크기차이를 이용한 세포 분리법이 기능성 세포 획득에 이용될 수 있음을 시사한다. 이와 같은 방법을 통해 다양한 종류의 기능성 세포 분리에 이용될 수 있을 것이라 생각된다.

보문 : 식품 및 환경, 기타 ; DGGE 방법과 Pyrosequencing 방법을 이용한 지렁이 장내미생물의 다양성 분석

김은성 ( Eun Sung Kim ) , 홍성욱 ( Sung Wook Hong ) , 정건섭 ( Kun Sub Chung )
4,500
초록보기
The beneficial effects of Eisenia fetida on soil properties have been attributed to their interaction with soil microorganisms. The bacterial diversity of the intestinal tract of E. fetida was investigated by culture-dependent and culture-independent methods including denaturing gradient gel electrophoresis (DGGE) and pyrosequencing analyses. In a pure culture, Lysinibacillus fusiformis (51%), Bacillus cereus (30%), Enterobacter aerogenes (21%), and L. sphaericus (15%) were identified as the dominant microorganisms. In the DGGE analyses, B. cereus (15.1%), Enterobacter sp. (13.6%), an uncultured bacterium (13.1%), and B. stearothermophilus (7.8%) were identified as the dominant microorganisms. In the pyrosequencing analyses, Microbacterium soli (26%), B. cereus (10%), M. esteraromaticum (6%), and Frigoribacterium sp. (6%) were identified as the dominant microorganisms. The other strains identified were Aeromonas sp., Pseudomonas sp., Borrelia sp., Cellulosimicrobium sp., Klebsiella sp., and Leifsonia sp. The results illustrate that culture independent methods are better able to detect unculturable microorganisms and a wider range of species, as opposed to isolation by culture dependent methods.

보문 : 식품 및 환경, 기타 ; Single-cathode와 Dual-cathode 미생물연료전지의 탄소원과 질산성질소의 전류발생 특성

장재경 ( Jae Kyung Jang ) , 이은영 ( Eun Young Lee ) , 유영선 ( Young Sun Ryou ) , 이성현 ( Sung Hyoun Lee ) , 황지환 ( Ji Hwan Hwang ) , 이형모 ( Hyung Mo Lee ) , 김종구 ( Jong Goo Kim ) , 강연구 ( Youn Koo Kang ) , 김영화 ( Young Hwa Kim )
4,500
초록보기
Microbial fuel cells (MFC), devices that use bacteria as a catalyst to generate electricity, can utilize a variety of organic wastes as electron donors. The current generated may differ depending on the organic matter concentrations used, when other conditions, such as oxidant supply, proton transfer, internal resistance and so on, are not limiting factors. In these studies, a single-cathode type MFC (SCMFC) and dual-cathode type MFC (DCMFC) were used to ascertain the current`s improvement through an increase in the contact area between the anode and the cathode compartments, because the cathode reaction is one of the most serious limiting factors in an MFC. Also an MFC was conducted to explore whether an improvement in electricity generation resulted from oxidizing the carbon sources and nitrates. About 250 mg L-1 sodium acetate was fed to an anode compartment with a flow rate of 0.326 mL min-1 by continuous mode. The current generated from the DCMFC was higher than the value produced from MFC with a single cathode. COD removal of dual-cathode MFC was also higher than that of single-cathode MFC. The nitrate didn`t affect current generation at 2 mM, but when 4 and 8 mM nitrate was supplied, the current in the single-cathode and dual-cathode MFC was decreased by 98% from 5.97±0.13 to 0.23±0.03 mA and 8.40±0.23 to 0.20±0.01 mA, respectively. These results demonstrate that increasing of contact area of the anode and cathode can raise current generation by an improvement in the cathode reaction.
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