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논문검색은 역시 페이퍼서치

한국미생물·생명공학회지검색

Microbiology and Biotechnology Letters


  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기: 계간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : - / SCOPUS
  • - ISSN : 1598-642x
  • - 간행물명 변경 사항 : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)
논문제목
수록 범위 : 40권 3호 (2012)

건축공학분야에서 탄산칼슘형성세균의 응용과 전망

박성진 ( Sung Jin Park ) , 김사열 ( Sa Youl Ghim )
5,100
초록보기
Microbiological calcium carbonate precipitation (MCCP) is being applied for the aesthetic restoration of cement buildings destroyed by biochemical processes and to block water penetration into the cement`s inner structure. After determining the advantages of this technique, many related studies in the area of architecture concerning the application of microorganisms to improve construction material have been reported in both America and Europe. The techniques compatibility with cement material is especially interesting because of the needed screening of various calcium carbonate forming-bacteria and the required development of their application methods. The purpose of this review is to describe the mechanism of MCCP and related researches with eco-friendly construction materials. Mainly, we describe the methodological studies focused on biodeposition on the surface of building materials and the research trends concerning the addition of microorganisms to improve the durability of cement structures. Additionally, the concepts and technical aspects focused on the development of self-healing smart concrete, with the use of multi-functional bacteria, have been considered.

제 2형 당뇨병 치료제의 개발 동향

이수현 , 이종근 , 김익환
4,500
초록보기
Type 2 Diabetes Mellitus, a chronic metabolic disorder which results from a high blood glucose level, is one of the most prevalent and costly diseases of our time. Considering increasing rates of obesity and the aging population in Korea, the number of diabetic patients is likely to rise rapidly in the future. There are five conventional diabetic drugs which work through different mechanisms; sulfonylureas, biguanide, meglitinide, alpha-glucosidase inhibitors, and thiazolidinedione. Although they all have antidia-betic effects, some side effects such as hypoglycemia, weight gain and gastrointestinal intolerance are associated with them. Incretin based therapies, utilizing glucagon-like peptide-1 (GLP-1) and dipeptidyl peptidase-4(DPP-4) inhibitors, which have a lower risk of adverse side effects, have recently been introduced. At present PPAR-targeting drugs are being actively developed. In this research review, particular emphasis has been placed on the current trends and possible biological targets for the new generation of antidiabetic drugs.
1,000
초록보기
A gene coding for mannanase (manA) from Bacillus subtilis was introduced into a shuttle vector pGK12 between Escherichia coli, B. subtilis and Lactobacillus paracasei. As a result of transferring the resultant plas-mid, designated pGK12M3, into three different strains, the manA gene was found to be expressed in L. paracasei as well as in B. subtilis and E. coli. In a 4 L fermentor culture, the production of mannanase by recombinant L. paracasei (pGK12M3) reached a maximum level of 5.4 units/ml in an MRS medium with a fixed pH 6.5. Based on the zymogram of mannanase, it is assumed that mannanase produced by recombinant L. paracasei is not maintained stably with proteolytic degradation. The optimal temperature and thermostability of mannanase produced by recombinant L. paracasei were also found to be different from those of enzymes produced by B. subtilis.

미생물제제시용 고추경작지로부터 식물생장홀몬과 항진균물질을 동시에 생산하는 식물생장촉진근권세균의 선발 및 동정

정병권 ( Byung Kwon Jung ) , 임종희 ( Jong Hui Lim ) , 안창환 ( Chang Hwan An ) , 김요환 ( Yo Hwan Kim ) , 김상달 ( Sang Dal Kim )
4,500
초록보기
In this study, a total of more than 1,000 bacteria, including 739 species of aerobic bacteria, 80 species of urease producing bacteria and 303 species of photosynthetic bacteria, were isolated from red-pepper field soils located in the Gyeongsan Province of the Republic of Korea. Amongst these, 158 species of aerobic bacteria, 70 species of urease producing bacteria and 228 species of photosynthetic bacteria were found to be auxin producing soil bacteria through quantification analysis with the Salkowski test. The latter groupings were then tested for antifungal activities to β-Glucanase and siderophore using CMC congo red agar and CAS blue agar media. In addition, the selected strains were examined for antifungal activity against various phytopathogenic fungi on PDN agar media. Six strains; BCB14, BCB17, C10, HA46, HA143, and HJ5, were noted for their ability to both produce auxin and act as antifungal substances. 16S rDNA sequence comparison analyses of these six strains identified them as Bacillus subtilis BCB14, B. methylotrophicus BCB17, B. methylotrophicus C10, B. sonorensis HA46, B. subtilis HA143, and B. safensis HJ5.
5,100
초록보기
This study investigated the role of plasmids and their relationship with the multiple antibiotic resistance of 30Vibrios sp. isolated from molluscs and crustaceans sampled from the Kerala coastal waters of India. The bio-chemical identification and antibiotic resistance profiles were determined, followed by the plasmid profiles, conjugation and transformation efficiencies. The results showed a considerable difference in the level of bacterial resistance to various antibiotics; while all 30 strains were found to be MAR Vibrios sp. and their resistance patterns varied. All the strains were resistant to amoxycillin, ampicillin and carbeniciliin. 87% were resistant to rifampicin; 74% to cefuroxime; 67 to streptomycin; 53% to norfloxacin and ciprofloxacin and 47% to furazolidone and nalidixic acid. In addition to their antibiotic resistance, the plasmid DNA of the MAR Vibrios strains isolated from the molluscs and crustaceans was also studied. Nine strains isolated from crustaceans and molluscs were found to harbor 1-3 plasmids with sizes varying from 5.98 kb to 19.36 kb. The average transformation efficiency was about 5×10(-8) and the conjugation efficiency varied from 2. 1×10(-3) to 10(-9). A further study of antibiotic resistance patterns may be useful to test the extent of drug resistance in seafoods andhelp to devise a nationwide antibiotic policy.

왕겨초액의 대량생산과 항노화 및 미백 효과

황재규 ( Jae Gyu Hwang ) , 윤종국 ( Jong Kuk Yun ) , 김숙경 ( Suk Kyung Kim ) , 이상한 ( Sang Han Lee ) , 한길환 ( Kil Hwan Han )
4,500
초록보기
Chaff-vinegar is known for having a variety of useful purposes in the fields of health and lifestyles. In a previous study we isolated and identified the active fractions of the polyphenol com-pound 7 species as a potential biomaterial for cosmeceuticals. To further test for its potential use as a functional material, we carried out an MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity assay and a xanthine oxidase inhibition assay. Chaff-vinegar exhibited potent collagenase and elastase inhibitory activities in a concentration dependent manner, indicating that the agent has the potential to alleviate the skin wrinkling process. Chaff-vinegar also showed80% tyrosinase inhibition at a concentration of 100μL/mL. DPPH radical scavenging, xanthine oxidase inhibition, and SOD-like activity results for each activity were 80%, 80%, and 100%, respectively. Taken together, the present study suggests that chaff-vinegar is a good candidate for use as an anti-wrinkling and/or whitening agent.

Resistance to Hypoosmotic Shock of Liposomes Containing Novel Pigments from an Antarctic Bacterium

( Correa Llanten ) , ( Daniela N. ) , ( Maximiliano J. Amenabar ) , ( Jenny M. Blamey )
4,500
초록보기
Although the antioxidant capacity of carotenoids and their role in regulating membrane fluidity have been well studied, their ability to confer resistance to hypoosmotic shock is poorly understood. In this work, we analyzed the effect of a mixture of carotenoid pigments obtained from an Antarctic microorganism belonging to the genus Pedobacter on liposomal resistance to hypoosmotic conditions. Intercalation of pigments intoliposomal structures resulted in an improvement of membrane resistance by decreasing the percentage of calcein released in comparison to that by liposomes without pigments. Due to these properties, such pigments could be useful for biotechnological applications.

카사바 당화액을 이용한 실험실용 및 산업용 효모의 에탄올 발효성능 비교

진영욱 ( Young Wook Chin ) , 김진우 ( Jin Woo Kim ) , 박용철 ( Yong Cheol Park ) , 서진호 ( Jin Ho Seo )
4,500
초록보기
In order to investigate the ethanol fermentation properties of alcohol yeasts a laboratorial strain (CEN.PK2-1D) and two industrial alcohol yeasts (JHS100 and JHS200) of Saccharomyces cerevisiae were cultured in a pure YP medium with 300 g/L glucose and cassava hydrolysate. Spot assay and cell viability tests showed that both the JHS100 and JHS200 strains exhibited higher ethanol tolerance than the CEN.PK2-1D strain. The JHS100 strain demonstrated the highest cell growth, glucose consumption and ethanol production. In particular, an anaerobic batch fermentation of the JHS100 strain using cassava hydrolysate with 250 g/L glucose resulted in a 106.1 g/L ethanol concentration, 0.42 g/g ethanol yield and 3.15 g/L-hr ethanol productivity, which were 53%, 13%, 53% higher than the corresponding values for the CEN.PK2-1D strain. By changing the pure YP medium to cassava hydrolysate, 19% and 17% decreases in ethanol yield and productivity for the CEN.PK2-1D strain were observed, whereas the cultures of the JHS100 and JHS200 stains showed similar ethanol pro-ductivities and only an 8% decrease in ethanol yield. Furthermore, the JHS100 and JHS200 stains produced lower levels of glycerol and acetate byproducts than the CEN.PK2-1D strain. Consequently, the outstanding ethanol fermentation performance of the industrial strains might be owing to rapid cell growth, high ethanol tolerance, low nitrogen requirements and the low formation of by-products.
초록보기
In the present work, the influence of prebiotic fructooli-gosaccharide (FOS) on adhesion to Caco-2 cells, viability, acid and bile tolerance, antibacterial, antioxidant, enzymatic, and metabolic activities of the probiotic starters Lactobacillus acidophilus GK20 and Lactobacillus paracasei GK74, has been explored. Experiments were conducted with fermented yoghurt over a period of 7 daysat 4˚C. When compared to control fermentations without prebiotic, the addition of FOS was seen to significantly(p<0.05) increase the viable cell counts of the probiotics, overall viscosity, and concurrently reduce the pH of the fermented yoghurts. Both Escherichia coli ATCC 11229 and Salmonella enteritidis ATCC 13076 were inhibited by the probiotics` antibacterial activities, while the synbiotic yoghurt containing mixed probiotics and FOS was noted to highly improve antagonistic action. When fermented with mixed starters, the addition of FOS (1.0%) resulted in the highest proteolytic (1.06±0.06 unit) and β-galactosidase activities (20.14±0.31 unit). However, FOS did notaffect acid and bile tolerance, adhesion to Caco-2 cells or the antioxidant activity of the probiotics, although both L.acidophilus GK20 and L. paracasei GK74 had functionality as probiotic strains. Hence, a significant symbiotic effect was observed in fermented yoghurt after 7 days of storage at 4˚C, and as a result, such synbiotic yoghurt can be said to possess synergistic actions which improve the gastrointestinal environment and promote of health.

비수리 추출물 함유 크림의 보습 효능 및 안정성 평가

이혜진 ( Hye Jin Lee ) , 구현아 ( Hyun A Gu ) , 박수남 ( Soo Nam Park )
4,500
초록보기
In this study, the skin moisturing effect and stability of cream containing L. cuneata G. Don extract (ethyl acetate fraction) were evaluated. The skin hydrating effect of the cream containing extract was 1020% higher than the placebo cream, and the TWEL of the cream containing extracts was decreased to 7.7 g/m2hcompared to the control (10.2 g/m2) and placebo cream (8.9 g/m2h). The pH, viscosity, and absorbance were measured under the 4, 25, 37, 45˚C and the sun light during the 12 weeks. The pH change between cream containing extract and placebo cream did not show the significant difference under the 4, 25, 37, 45˚C except for the sun light. Both creams showed high decrease (about 59%) of viscosity at 45˚C. However, there was no significant change under other conditions. The absorbance of the cream containing the extract and the placebo cream was decreased similarly at all conditions. This decrease in absorbance was relatively small compared to the decrease of absorbance of the extract in ethanol solution under the sun light (Fig. 7). In addition, any change in color or smell of the cream was not observed during the 12 weeks. Also physical changes as creaming and cohesion were not shown. These results indicate that the cream containing L. cuneata extract has the skin moisturizing effect and is relatively stable. Therefore, it is suggested that the ethyl acetate fraction of L. cuneata extract could be applicable to cosmetics as a new cosmetic material with its antioxidative and antibacterial activities reported previously.
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