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논문검색은 역시 페이퍼서치

한국미생물·생명공학회지검색

Microbiology and Biotechnology Letters


  • - 주제 : 자연과학분야 > 생물
  • - 성격 : 학술지
  • - 간기: 계간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : - / SCOPUS
  • - ISSN : 1598-642x
  • - 간행물명 변경 사항 : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)
논문제목
수록 범위 : 41권 3호 (2013)

용인 함박산 토양에서 분리한 Paenibacillus sp. HX-1의 동정과 endo-β-1,4-xylanase 생산 증가를 위한 배지최적화

지원재 ( Won Jae Chi ) , 김종희 ( Jong Hee Kim ) , 홍순광 ( Soon Kwang Hong )
4,500
초록보기
A xylanase-producing bacterium was isolated from a soil sample collected in Yongin city, Korea. The strain was aerobic and gram positive, and grew between pH 5.0 and 11.0, forming a yellow-colored colony. The strain was classified as a novel subspecies bacterium of Paenibacillus barcinonensis by 16S rRNA gene sequence similarity, phylogenetic analysis, phenotypic, and biochemical characteristics, and thus named Paenibacillus sp. HX-1. This strain produced extracellular endo-β-1,4-xylanase, and the best xylanolytic activity (205.17 unit/ml) was obtained at 96 h in an optimized TNX medium containing 1% (w/v) bacto tryptone, 1% (w/v) NaCl, and 0.7% (w/v) beechwood xylan at pH 7.0, 37oC and 200 rpm. The endo-β-1,4-xylanase produced by the strain HX-1 yielded xylobiose as the end product from beechwood xylan hydrolysis. The enzyme exhibited optimum pH and temperature at pH 7.0 and 45oC, respectively. The remarkable enhancing effect of the TNX medium on xylanase production by HX-1, in spite of its simple formula, may have great advantages for industrial applications of xylanase.

주목 식물세포(Taxus chinensis) 배양 유래 타르 성분 동정 및 정량

김건중 ( Gun Joong Kim ) , 박규연 ( Gyu Yeon Park ) , 김진현 ( Jin Hyun Kim )
4,500
초록보기
In this study, the tar compounds derived from the plant cell cultures of Taxus chinensis were first identified and then quantified via gas chromatography/mass spectrometry (GC/MS) and gas chromatography (GC). 2-Picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene and o-xylene were found to be the major tar compounds by biomass. These compounds were identified and confirmed by comparing their retention times with those of authentic compounds. Each compound also spiked with the pure standard. The contents of 2-picoline, 2,5-xylenol, acenaphthene, 1-methylnaphthalene, and o-xylene in biomass were 0.2512, 0.1586, 0.1240, 0.0942 and 0.0525 wt%, respectively. Liquid-liquid extraction and adsorbent treatment were able to remove 42% and 94% of the tars from biomass, respectivly. After hexane precipitation, all of the tars were perfectly removed.

Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven

( Correa Llanten Daniela ) , ( Juanita Larrain Linton ) , ( Patricio A Munoz ) , ( Miguel Castro ) , ( Freddy Boehmwald ) , ( Jenny M Blamey )
4,500
초록보기
A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between 60-80ºC (optimum 70ºC), in a pH range of 3.0-8.0 (optimum pH70ºC 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(ω7) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA?DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.

삼지구엽초 추출물의 항균 및 항산화 활성에 대한 연구

원두현 ( Doo Hyun Won ) , 구현아 ( Hyun A Gu ) , 김혜진 ( Hye Jin Kim ) , 한샛별 ( Saet Byeol Han ) , 박진오 ( Jin Oh Park ) , 박수남 ( Soo Nam Park )
4,500
초록보기
In this study, the antibacterial and antioxidative activities of Epimedium koreanum Nakai were investigated for applications as cosmetic ingredients. Minimum inhibitory concentrations (MICs) of fraction-bacterium, that showed high antibacterial activity from disc diffusion assay on human skin pathogens, were tested. The ethyl acetate fraction on Staphylococcus aureus, Bacillus subtilis, Propionibacterium acnes and 50% ethanol extract on S. aureus exhibited higher antibacterial activities than methyl paraben, well known as a preservative. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities of 3 fractions of E. koreanum Nakai were lower than (+)-α-tocopherol, known as a typical antioxidant. From the results of the scavenging activities of various ROS generated in Fe3+-EDTA/H2O2 systems (OSC50), 50% ethanol extract (OSC50 = 2.46 ± 0.06 μg/ml) and aglycone fraction (OSC50 = 1.45 ± 0.02 μg/ml) showed high activities similar to L-ascorbic acid (OSC50 = 1.50 ± 0.85 μg/ml), used as reference. The cellular protective effects (τ50) on photohemolysis by 1O2 generated by photosensitization reaction were tested. The cellular protective effect of 50% ethanol extract (τ50 = 37.0 ± 0.3 min) was similar to (+)-α-tocopherol (τ50 = 38.0 ± 1.8 min), used as reference. In particular, the τ50 of aglycone fraction results were 165.9 ± 7.2 min. This is a high cellular protective effect, more than 4 times that of (+)-α-tocopherol. These results indicate that E. koreanum Nakai extract, and its fractions, could be utilized as a cosmetic ingredient possessing antibacterial and antioxidative activities.

PCR-DGGE를 이용한 친환경 농법 적용 고추경작지 내 진균의 군집 다양성 분석

정병권 ( Byung Kwon Jung ) , 김광섭 ( Gwang Seop Kim ) , 송진하 ( Jin Ha Song ) , 김상달 ( Sang Dal Kim )
4,500
초록보기
The coastal sand-dune plants of eight species; Argusia sibirica, Calystegia soldanella, Elymus mollis, Lithospermum zollingeri, Raphanus sativus, Salsola collina, Zoysia macrostachya, and Zoysia sinica were collected from the Shindu-ri coastal sand dune. Ninety-eight endophytic fungal strains were isolated from the roots of these plants, analyzed, and identified by sequences in their internal transcribed spacers (ITS) at the ITS1, 5.8S, and ITS2 regions. The diversity of endophytic fungi isolated from coastal sand-dune plants was confirmed with various diversity indices. The fungal strains belonged to thirteen orders: Capnodiales (3.09%), Eurotiales (70.10%), Glomerellales (1.03%), Helotiales (3.09%), Hypocreales (9.28%), Mortierellales (2.06%), Onygenales (1.03%), Ophiostomatales (1.03%), Pleosporales (1.03%), Polyporales (1.03%), Russulales (1.03%), Saccharomycetales (2.06%), and Xylariales (1.03%). Of the endophytic fungal strains collected, Penicillium (59.18% in Eurotiales) and Fusarium (5.10% in Hypocreales) were the most abundant in coastal sand-dune plants. The endophytic fungal strains isolated from C. soldanella were more diverse compared to strains from the other coastal sand-dune plants

신두리 해안사구에 자생하는 사구식물 내생진균의 다양성 분석

유영현 ( Youn Hyun You ) , 서영교 ( Yeong Gyo Seo ) , 윤혁준 ( Hyeok Jun Yoon ) , 김현 ( Hyun Kim ) , 김예은 ( Ye Eun Kim ) , 이리나할무라토바1 ( Irina Khalmuratova ) , 임순옥 ( Soon Ok Rim ) , 김창무 ( Chang Mu Kim ) , 김종국 ( Jong Guk Kim )
5,100
초록보기
The coastal sand-dune plants of eight species; Argusia sibirica, Calystegia soldanella, Elymus mollis, Lithospermum zollingeri, Raphanus sativus, Salsola collina, Zoysia macrostachya, and Zoysia sinica were collected from the Shindu-ri coastal sand dune. Ninety-eight endophytic fungal strains were isolated from the roots of these plants, analyzed, and identified by sequences in their internal transcribed spacers (ITS) at the ITS1, 5.8S, and ITS2 regions. The diversity of endophytic fungi isolated from coastal sand-dune plants was confirmed with various diversity indices. The fungal strains belonged to thirteen orders: Capnodiales (3.09%), Eurotiales (70.10%), Glomerellales (1.03%), Helotiales (3.09%), Hypocreales (9.28%), Mortierellales (2.06%), Onygenales (1.03%), Ophiostomatales (1.03%), Pleosporales (1.03%), Polyporales (1.03%), Russulales (1.03%), Saccharomycetales (2.06%), and Xylariales (1.03%). Of the endophytic fungal strains collected, Penicillium (59.18% in Eurotiales) and Fusarium (5.10% in Hypocreales) were the most abundant in coastal sand-dune plants. The endophytic fungal strains isolated from C. soldanella were more diverse compared to strains from the other coastal sand-dune plants

안동지역 종가에서 전통적으로 제조된 간장 장아찌의 품질 특성

김덕진 ( Deok Jin Kim ) , 김미선 ( Mi Sun Kim ) , 이예슬 ( Ye Seul Lee ) , 손호용 ( Ho Yong Sohn )
4,500
초록보기
In an effort to characterize the physicochemical properties and microbial risks associated with the soy sauce jangachi (Korean traditional pickle), 15 different home-made products, which were prepared from medicinal plants and wild edible vegetables, from head-families of Andong, Kyungsangbuk-do Province in Korea, and 6 different commercial products sold at supermarket, were investigated. The average pH of the mature soaking solutions and plants soaked in the 21 jangachi were 3.99 ± 0.38 and 3.51 ± 0.41, and the average acidity of the mature soaking solutions and soaked plants were 1.59 ± 0.54 and 1.65 ± 0.76, respectively. The average brix of the mature soaking solutions and plants soaked were 27.67 ± 8.38 and 25.61 ± 6.60, respectively. In salinity, which is a major factor in jangachi industry production, the average salinity of the mature soaking solutions and soaked plants were 7.55 ± 3.26 and 5.75 ± 2.23, respectively. In particular, the hot-peppers, eusuri, du-rup, kaet-ip, kuji-ppong, myeng-i and sancho jangachi were amongst the home-made products, and the salinity was above 8.8%, which was 2 foldshigher than that of the commercial sterilized products, and 1/3-lower than commercial non-sterilized products. The color difference and turbidity of jangachi were dependent on the plant parts used. In microbial risk assessment, the microorganisms related with food-borne disease, such as Escherichia coli, Salmonella sp, and Shigella sp., were not detected. After some time, total cell count analysis revealed that the commercial products sold at supermarkets were more vulnerable than the homemade products.

피부 흡수 증진을 위한 담쟁이덩굴 줄기 추출물 함유 나노에멀젼 및 이의 항균활성 연구

조나래 ( Na Rae Jo ) , 박민아 ( Min A Park ) , 전소하 ( So Ha Jeon ) , 박수남 ( So Ha Jeon )
4,500
초록보기
In a previous study, we investigated the antioxidative and cellular protective effects of Parthenocissus tricuspidata stem extracts. In this study, we prepared nano-emulsion containing P. tricuspidata stem extract to improve skin permeation. The particle size of the nano-emulsion using the microfluidizer was 302 nm. Its loading efficiency was over 86%. The size distribution of the nano-emulsion took a monodispersed form and the nano-emulsion was more stable than typical emulsion without using microfluidizer during a 2 week period. In vitro skin permeation study of nano-emulsion containing P. tricuspidata stem extracts was carried out using Franz diffusion cell. The 1,3-butylene glycol used as a control group had 32.59% skin permeation efficiency. The skin permeation efficiency of the nano-emulsion was 42.47%. Also, we observed the antibacterial activity of the ethyl acetate fraction on skin flora for prospective applications as a natural antimicrobial. The ethyl acetate fraction had antibacterial activities higher than methyl paraben on Staphylococcus aureus, and Bacillus subtilis. These results indicate that nanoemulsion containing P. tricuspidata stem extracts could possess valued applications in cosmetic formulations for improving skin permeation. Also, based on the antibacterial activities on skin flora, antioxidative and cellular protective effects shown in our previous study, we suggest that P. tricuspidata stem extracts could be used as functional cosmetic materials.

다래를 이용한 발효주의 제조 및 이화학적 특성

박경록 ( Kyung Lok Park ) , 홍성욱 ( Sung Wook Hong ) , 김영준 ( Young Joon Kim ) , 김수재 ( Soo Jae Kim ) , 정건섭 ( Kun Sub Chung )
4,500
초록보기
For the development of hardy kiwi wine, we arranged for the post-maturity of hardy kiwi fruit, treated them with calcium carbonate and a pectinase enzyme complex, investigated the resulting physicochemical properties and conducted a sensory evaluation. The period determined for creating post-maturity in the hardy kiwi fruit was determined as 5 days storage at room temperature following maturity. During this time the yield of fruit juice was increased from 22.1% to 53.5% using 0.1% (v/v) cytolase PCL5 for 2 h at room temperature. 0.1% (w/v) calcium carbonate was also added during the process of aging, for the reduction of the sour taste. The fermentation trial of the hardy kiwi wine was prepared using water (25% or 50%), sugar (24 ºbrix), 0.1% (w/v) CaCO3, 0.1% (v/v) cytolase PCL5, K2S2O5 (200 ppm), and yeast (1.5 × 107 cell/ml). Fermentation then occurred for 2 weeks at 20oC. The pH value, total acidity, alcohol, and reducing sugar content of the resulting hardy kiwi wines of 25% (v/w) and 50% (v/w) water, were in a range of pH 3.4-3.7, 1.12-1.21%, 14.3-14.4%, and 15-16 g/l, respectively. Citric acid and fructose constituted the major organic acids and the free sugar of the 25% and 50% hardy kiwi wine, respectively. Volatile flavor components, including 10 kinds of esters, 8 kinds of alcohols, 5 kinds of acids, 3 kinds of others and aldehydes, were determined by GC analysis. The results of sensory evaluation demonstrated that 50% hardy kiwi wine is more palatable than 25% hardy kiwi wine.

Antimicrobial Effect of Inula britannica Flower Extract against Methicillin-resistant Staphylococcus aureus

( Na Kyoung Lee ) , ( Jang Hyun Lee ) , ( Yong Ju Lee ) , ( Sin Hye Ahn ) , ( Su Jin Eom ) , ( Hyun Dong Paik )
4,500
초록보기
The antimicrobial effect of the methanol extract of Inula britannica flowers against methicillin resistant Staphylococcus aureus (MRSA) was investigated. It was confirmed that the methanol extract is mainly composed of quercetin, which has antimicrobial properties. The antimicrobial effect of the methanol extract against 3 MRSA strains was determined by the disc diffusion method. The minimal inhibitory concentrations were ranged from 0.625 mg/ml to 1.25 mg/ml, and the minimum bactericidal concentrations were 2.5 mg/ml. Time kill kinetics revealed bactericidal activities, and the morphological alterations in S. aureus ATCC 33591 treated with the extract were observed using a scanning electron microscope. The methanol extract affected the expression of the resistant genes, mecA, mecI, and mecRI in mRNA. Therefore, the methanol extract of I. britannica flowers clearly demonstrated an antimicrobial effect against MRSA and these results suggest a potential for application as a natural antimicrobial agent.
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