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> 생화학분자생물학회 > BMB Reports > 34권 3호

Short Communications : Inactivation of Brain Glutamate Dehydrogenase Isoproteins by MDL 29951

Short Communications : Inactivation of Brain Glutamate Dehydrogenase Isoproteins by MDL 29951

(Eun Young Lee) , (Hye Young Yoon) , (Tae Ue Kim) , (Soo Young Choi) , (Moo Ho Won) , (Sung Woo Cho)

- 발행기관 : 생화학분자생물학회

- 발행년도 : 2001

- 간행물 : BMB Reports, 34권 3호

- 페이지 : pp.268-273 ( 총 6 페이지 )


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논문제목
초록(외국어)
In addition to the recognition site for glutamate, the Nmethyl-D-aspartate (NMDA)-preferring glutamate receptor subtype shows a binding site for glycine. In this paper, we present the effects of 3-(4,6-dichloro-2-carboxymethylamino-5,7-dichloroquinoline-2-carboxylic acid (MDL 29951), a potent inhibitor of glycine binding to the NMDA receptor, on glutamate dehydrogenase (GDH) from bovine brains. The incubation of GDH isoproteins from bovine brains with MDL 29951 resulted in a dose-dependent loss of enzyme activity Separately or together, 2-oxoglutarate and NADH did not give an efficient protection against the inhibition, indicating that GDH isoproteins saturated with NADH or 2-oxoglutarate are still open to attack by MDL 29951. MDL 29951 was an uncompetitive inhibitor with respect to both 2-oxoglutarate and NADH for GDH isoproteins. These results suggest that the binding site of MDL 29951 is not directly located at the catalytic site, and the inhibition of GDH isoproteins by MDL 29951 is probably due to a steric hindrance, or a conformational change altered upon the interaction of the enzyme with its inhibitor. The inhibitory effects of MDL 29951 on GDH isoproteins were significantly diminished in the presence of ADP GDH I reacted more sensitively with ADP than GDH B on the inhibition by MDL 29951. Our results suggest a possibility that the two types of GnHs are differently regulated by MDL 29951, depending on the physiological concentrations of ADP.

논문정보
  • - 주제 : 자연과학분야 > 화학
  • - 발행기관 : 생화학분자생물학회
  • - 간행물 : BMB Reports, 34권 3호
  • - 발행년도 : 2001
  • - 페이지 : pp.268-273 ( 총 6 페이지 )
  • - UCI(KEPA) : I410-ECN-0102-2009-470-006066091
저널정보
  • - 주제 : 자연과학분야 > 화학
  • - 성격 : 학술지
  • - 간기 : 월간
  • - 국내 등재 : KCI 등재
  • - 해외 등재 : - / SCOPUS
  • - ISSN : 1976-6696
  • - 수록범위 : 1968–2022
  • - 수록 논문수 : 4632