Objectives: The purpose of this study is to develop a performance measurement tool for public health center information system and to apply the tool in evaluating the information systems currently in operation. Methods: We designed a performance measurement model of public health center information system using IS-BSC conceptual framework which consists of 4 perspectives; Business Value Perspective, User Orientation Perspective, Internal Process Perspective and Future Readiness Perspective. Within the framework we developed a performance measurement tool consisted of 35 questionnaires and examined its validity by administrating a survey to the system users. Results: The validity of the performance evaluation tool was supported by the results of statistical analysis such as factor analysis, Chronbach`s α test and regression analysis. The relative importance of the 4 performance perspective was analyzed by regressing the perspectives` scores on two overall performance indicators; a job performance indicator and an organization performance indicator. The results show that impact of business value perspective to job performance and the impact of future performance were the primary determinant. Conclusions: The public heath center information system performance measurement tool suggested was found to be conceptually sound and methodologically valid. Also, based on the result of performance evaluation on current information system, we suggest more investment to the education and training of heath center personnel of the system utilization.