Objectives. Proper cleansing of dentures is important for oral mucosal health in the elderly. We evaluated denture cleansers using in vitro antimicrobial tests with Candida albicans and Streptococcus mutans, as well as a denture base abrasion test. Methods. 1) We administered a questionnaire about denture cleansers to 632 elderly people in Daejeon. 2) The dentifrices, kitchen detergents were selected as artificial denture cleansers, and baking soda and vinegar as natural denture cleansers. Antimicrobial tests were performed by spectrophotometry using a McFarland nephelometer and total plate counts after incubation with each material. 3) Denture base resin discs (13×1 mm) were prepared for abrasion tests using a V8 Crossbrushing machine. Results. 1) Most elderly people used the dentifrices as denture cleansers was 65.8%. 2) After Vinegar treatment was less than 10% effective in bacteriostatic and bacteriocidal tests of S. mutans and antifungal effects of C. albicans. The antimicrobial effect of baking soda and dentifrices depended on the dilution ratio. Kitchen detergents showed an inverse relationship between dilution ratio and antimicrobial effect. 3) The dentifrices caused the most abrasion, but the effect was not significant. Conclusions. Vinegar and baking soda could be useful denture cleansers, but must be tested on other denture materials.