Background: Rosa multiflora Thunberg fruit has been traditionally used as a dietary supplement and a herbal remedy for the treatment of various diseases, including inflammation, osteoarthritis, rheumatoid arthritis and chronic pain. We found that Rosa multiflora Thunberg fruit extracts have therapeutic effect on photo-aging skin without remarkable epidermal side-effects through PPAR メ/ャ dual agonistic effect. Objectives: We aim to evaluate the anti-wrinkle effect and the safety of Rosa multiflora Thunberg fruit extracts. Methods: Thirty three subjects were recruited and randomly applied with active cream (treated site) or vehicle cream (control site) to the left or right side of the face twice a day for 8 weeks. The active cream contains the active ingredients of Rosa multiflora Thunberg fruit extracts, while the vehicle cream has no active ingredients. Results: All subjects were women aged between 30 and 58 with the average age of 45.4 years. In gross evaluation, 12 subjects showed better results in the treated sites, whereas 6 subjects in the control sites. Based on clinical pictures, significant wrinkle reduction rate was observed in the treated sites. Analysis of replica showed the decrease in mean length of wrinkles and mean shape factor of wrinkles in the treated sites after 8 weeks. There were no remarkable side effects related with applied creams. Conclusion: Topical application of Rosa multiflora Thunberg fruit extracts could improve facial wrinkles without remarkable side-effects.