Objectives: To evaluate effects of an intervention using lay health advisors on promoting cancer screening and to develop effective strategies and health services. Methods: The number of subjects included in the final analysis was 188. Of those, 72 were applied to health services provided by public health centers (control group) and 116 composed the experimental group. They were selected from residents of Daegu and Gyeonsangbuk-do aged older than 40 years. We designed a questionnaire applying Trans-theoretical Model and Health Belief Model in order to determine the subjects’ intentions to participate in the national cancer screening program. Any thought changes occurred after the interventions were compared with the intial responses. For statistical analysis, paired t-test, ANCOVA and multiple logistic regression analyses were conducted. Results: After the intervention using lay health advisors, knowledge about cancer screening and its benefits were improved and self-efficacy increased with statistical significance. Moreover, barriers to cancer screening decreased. Compared to application of health services provided by public health centers, encouraging cancer screening by lay health advisors was 1.64 times more likely to generate forward stage movement in cancer screening, but there was no statistical significance. Conclusions: Even though, effects of the intervention using lay health advisors on promoting cancer screening were not clearly determined, it is a precedent study for emphasizing application of community resources and participation of residents in promoting cancer screening. Further studies are required to specify effects of the intervention.