Testosterone and dihydrotestosterone(DHT) that are affect hair loss. 5α -reductases are converting testosterone into DHT. In hair loss processes, TGF-β1, DKK-1, DHT/DHT-AR(androgen receptor) complex are key factors. RE-ORGA was extracted from natural ingredient to alleviate hair loss with less adverse effects. This study showed that RE-ORGA can induce proliferation of dermal papilla and regulate molecular signaling pathway. Western blot analysis treated with RE-ORGA showed lowered expression of 5α-reductases. From RNA-Seq their expression was also examined to be decreased in transcriptional levels. Log-scaled fold changes of SRD5A1,3 and Bax were -0.518, -0.253, -0.758. Androgen receptor and TFGB1I1, co-activator for androgen receptor, were also down-regulated. On the other hand Ptk2b that is negative androgen receptor regulator showed over-expression. These results showed that RE-ORGA could regulates hair loss induced by testosterone and DHT.