Objectives: We investigated relation between HDL-cholesterol and the incidence of impaired fasting glucose in obese and normal body weight by gender in Korean adults. Methods: 6,438 subjects aged 40-59 years (2,197 impaired fasting glucose, 4,241 normoglycemia) were selected except the patients with cardiovascular disease and pregnant women using 2016 KNHANES data. We analyzed the relation of impaired fasting glucose and HDL-cholesterol by BMI. Results: In both men and women regardless of BMI, who with low HDL-cholesterol significantly higher risk of impaired fasting glucose than who with normal HDL-cholesterol in unadjusted model. In except men with obesity, who with low HDL-cholesterol significantly higher risk of impaired fasting glucose than who with normal HDL-cholesterol when age, smoke, drink, exercise adjusted. In addition, women with normal BMI (OR, 1.82; 95% CI, 1.42-2.33) higher risk of impaired fasting glucose than women with obesity (OR, 1.57; 95% CI, 1.14-2.17). Conclusions: It is necessary to management of HDL-cholesterol to control impaired fasting glucose, except for obese men.