Objectives: The purpose of this study was to contribute to the improvement of hospital management by understanding the relationship between job stress and job performance and providing systematic and professional basic data. Methods: The research method examines the general characteristics (8 items) of the hospital staff and identifies the relationship between job stress (43 items), job satisfaction (5 items), and job performance (7 items). Data collected through the questionnaire (self-administration survey) were analyzed using reliability, frequency analysis and descriptive statistics, mean, independent sample (t-test), one-way ANOVA, correlation, and hierarchical regression analysis. Results: The results of the study showed that job stress according to the general characteristics of the research subjects was not significant in job satisfaction and job performance. Job satisfaction was found to be related to job stress, job instability, and compensation inadequacies among job stress factors. In addition, the relationship conflicts and job instability among the job stress factors were found to have a significant relationship with job performance, and job satisfaction was found to affect job performance. Conclusions: In order to increase job satisfaction and job performance of the medical institution workers, it is necessary to identify those who have a high level of job stress among general characteristics group and to provide professional and systematic management to reduce job stress in order to encourage them to demonstrate their abilities to the fullest extent.