Biorenovation modifies the structure of organic compounds, and thereby improving their biological properties. The current study aimed to examine the effects of Prunus yedoensis flowers (PYF) extract and the P. yedoensis flowers biorenovate (PYFB) extract on collagen synthesis and expression. Cytotoxicity on human dermal fibloblasts (HDF) was determined by cell viability assay kit (WST-8) assay. COL1A1, COL3A1, COL4A1 and elastin mRNA expression were confirmed by quantitative real time PCR. In addition, COL1A1, COL3A1 and elastin protein expression were confirmed by western blot. In vitro assays using the human dermal fibroblasts cells indicated that both PYF and PYFB extracts induced collagen synthesis. Quantitative real time PCR analysis showed that the treatment of these cells with the two extracts up-regulated the expression of COL1A1, COL3A1, COL4A1 and elastin factor genes, which are known to play a role in collagen synthesis. Notably, the effects of the PYFB extract were superior to that of the PYF extract. Therefore, our study suggests that PYFB extract could be developed as cosmetic ingredients with anti-aging effects that are better than PYF.