In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and anti-inflammation properties and can be used as a natural ingredient of cosmetic materials.