The purpose of the present study is to explore the factors that influence MZ generation's acceptance of YouTube-based beauty health information. A survey was conducted among 307 participants of MZ generation, and the main results were derived through exploratory factor analysis, reliability analysis, correlation analysis, and path analysis using SPSS 21.0 program and AMOS 21.0 program. The main results are as follows. First, optimism and innovativeness of the MZ generation influenced positively on perceived ease of use of YouTube-based beauty health information. On the other hand, discomfort and insecurity of the MZ generation influenced negatively on perceived ease of use and perceived usefulness of YouTube-based beauty health information. Next, perceived ease of use influenced positively on perceived usefulness, whereas, both perceived ease of use and perceived usefulness influenced positively on usage intention. In conclusion, to increase the use of beauty health information from YouTube by the MZ generation, it is necessary to promote actively the use of YouTube-based beauty health information, and apprise that no anxiety element is present in YouTube related technology.