The purpose of this study was to examine an association of lower extremity injury incidence and femoral muscle strength imbalance in female university athletes. Female athletes (N=132) participated in this study and their sports types were kendo (n=6, 4.5%), gymnastics (n=11, 8.3%), wrestling (n=2, 1.5%), badminton (n=6, 4.5%), boxing (n=1, 0.8%), basketball (n=12, 9.1%), judo (n=27, 20.5%), table tennis (n=9, 6.8%), taekwondo (n=37, 28%), fencing (n=8, 6.1%), and field hockey (n=13, 9.8%). This study investigated lower extremity injury incidence focusing on muscle imbalance during previous training and competition period of last 6 months. Lower extremity injury incidence during training period was 33 (25%) and during competition period was 41 (31%) and lower extremity injury incidence during competition period was significantly higher compared to upper extremity injury incidence (p=.046). Right and left hamstring to quadriceps ratio (H:Q ratio) did not show a significant association with injury incidence during training and competition period. However, lower extremity injury incidence induced by femoral muscle imbalance was significantly increased as 3.9 times (Confidence Interval(CI): 1.52 to 9.97) during competition and 2.8 times (CI: 1.01 to 7.53) during training period based on H:Q ratio. It would be needed to consider dominant leg H:Q ratio and right and left leg H:Q difference as well for preventing lower extremity injury in female athletes.